In order to analyze the composition, abundance, and diversity of hyperiid amphipods at Punta Cruces and Cabo Marzo, on the northern Pacific coast of Colombia (eastern tropical Pacific), a sampling campaign was carried out in January 2008 that covered a nine-station sampling grid. Twenty species were found, of which Lestrigonus bengalensis and Hyperioides sibaginis were the most abundant (91% of the community at Cabo Marzo and 95% at Punta Cruces). Although the abundance and diversity were highly variable at both sites, they did not differ significantly (Mann Whitney; p > 0.05). Likewise, the similarity in terms of composition and abundance between the hyperiid communities at Punta Cruces and Cabo Marzo was 64.6%. This research provides new information regarding a scarcely studied component of the zooplankton in the eastern tropical Pacific and increases the number of hyperiid species reported for the Pacific Ocean of Colombia.