A study of the diet of Hatcheria macraei caught in Chubut River, Bajada del Cóndor (43°31'21,12’’S; 69°06’22,1’’W) was performed in April 2000. The feeding regime of this Trichomycteridae agreed with the carnivorous bentophagous type, and the dominant preys of both adults and juveniles were chironomid larvae, tricopter larvae, and cladocerans. The adult specimens exhibited a more diverse diet than the juveniles.