Metabolic and osmoionic effects of the recombinant crustacean hyperglycemic hormone (rCHH-A) of the Pacific white shrimp Penaeus vannamei on specimens acutely exposed to extreme salinities

Verónica Balderas-González, Elizabeth Ponce-Rivas, Fernando Díaz, Laura Camacho-Jiménez, Edna Sánchez-Castrejón, Manuel B. Aguilar

Submited: 2022-07-13 17:31:35 | Published: 2023-04-30 20:21:48

DOI: https://doi.org/10.3856/vol51-issue2-fulltext-2967

Abstract


The crustacean hyperglycemic hormone (CHH) is a multifunctional neuropeptide that plays a central role in crustacean metabolism and physiology. Experiments were conducted to examine the metabolic and osmoionic capabilities of the white shrimp Penaeus vannamei injected with its recombinant CHH-A (rCHH-A) peptide and acutely transferred from iso- (26 ppt) to hypo- (10 ppt) and hyper-osmotic (40 ppt) conditions. Hemolymph glucose, protein, osmoregulatory capacity (OC), and ionoregulatory capacity (IC) for sodium, chloride, potassium, and calcium were evaluated at four post-injection times (0.5, 1, 2, and 24 h). The rCHH-A peptide had hyperglycemic activity in all salinity conditions, obtaining maximum values at 1 h post-injection. However, in shrimp transferred to hyper-osmotic condition, rCHH-A caused the most significant reductions in OC (2 h), chloride IC (2 h), and total proteins (0.5-2 h) compared to the phosphate-buffered saline (PBS) control. Contrastingly, in shrimp transferred to hypo-osmotic conditions, rCHH-A decreased OC significantly from 2-24 h, strongly decreased chloride and potassium IC at 1 h post-injection, and increased total protein concentration in the hemolymph (1-2 h) when compared to PBS control. Concerning calcium, the rCHH-A injection decreased calcium IC at 10 ppt (1 h) and 26 ppt (2 h), providing insight into a potential role for CHH-A in calcium regulation. The results suggest that glucose and protein mobilization could enhance energy for osmo-ionic regulation under extreme osmotic conditions. This research study contributed to understanding crustacean endocrinology in P. vannamei and related euryhaline crustaceans. Further research should be performed to understand the osmo- and ionoregulatory mechanisms of the different CHH variants in crustaceans exposed to other stress conditions and the relationship with intermediary energy metabolism regulation.


Balderas-González V, Ponce-Rivas E, Díaz F, Camacho-Jiménez L, Sánchez-Castrejón E, Aguilar M. Metabolic and osmoionic effects of the recombinant crustacean hyperglycemic hormone (rCHH-A) of the Pacific white shrimp Penaeus vannamei on specimens acutely exposed to extreme salinities. Lat. Am. J. Aquat. Res.. 2023;51(2): 248-267. Available from: doi:10.3856/vol51-issue2-fulltext-2967 [Accessed 18 Apr. 2024].
Balderas-González, V., Ponce-Rivas, E., Díaz, F., Camacho-Jiménez, L., Sánchez-Castrejón, E., & Aguilar, M. (2023). Metabolic and osmoionic effects of the recombinant crustacean hyperglycemic hormone (rCHH-A) of the Pacific white shrimp Penaeus vannamei on specimens acutely exposed to extreme salinities. Latin American Journal of Aquatic Research, 51(2), 248-267. doi:http://dx.doi.org/10.3856/vol51-issue2-fulltext-2967