Methionine sources and Bacillus amyloliquefaciens CECT 5940 effects on growth, body composition, and nutrient metabolism of Penaeus vannamei fed reduced fishmeal diets

Karla Janeth Arevalo-Sainz, José Reyes Gonzalez-Galaviz, Ramón Casillas-Hernández, Aldo Fraijo-Valenzuela, Juan Carlos Gil-Núñez, Libia Zulema Rodriguez-Anaya, Fernando Lares-Villa, Pablo Gortares-Moroyoqui, Joe Luis Arias-Moscoso, Rafael Apolinar Bórquez-López

Submited: 2023-10-02 20:35:16 | Published: 2024-06-30 20:15:52

DOI: https://doi.org/10.3856/vol52-issue3-fulltext-3158

Abstract


An eight-week feeding trial investigated the effects of fishmeal (FM) replacement by soybean meal (SBM) and poultry by-product meal (PBM) in diets supplemented with two methionine sources (DL-Met and AQUAVI®) and their combinations with ECOBIOL® (Bacillus amyloliquefaciens CECT 5940) on growth performance, body composition, and nutrient metabolism of Penaeus vannamei. Four hundred shrimp (0.30 ± 0.04 g) randomly distributed into 20 tanks (20 shrimp tank-1) were fed with five experimental diets (four repetitions per diet). A control diet (CD; 20% FM) and four diets with 50% FM replacement supplemented with different methionine sources and ECOBIOL® combinations: D1 (0.13% DL-MET), D2 (0.06% AQUAVI®), D3 (0.13% DL-MET plus 0.10% ECOBIOL®), and D4 (0.06% AQUAVI® plus 0.10% ECOBIOL®). Shrimp fed D2 and D4 had better growth performance, nutrient utilization, and body composition. Shrimp fed D3 also had good growth performance but higher lipid body composition. Shrimp fed D1 had the worst growth performance, nutrient utilization, and body composition values. Nutrient metabolism-related genes were affected by dietary treatments. Protein synthesis-related genes decreased mostly in shrimp fed D1 and D2 while increased mostly in shrimp fed D3 and D4, lipolysis-related genes had a better transcriptional response in shrimp fed D4 and lipogenesis-related genes were mostly downregulated in shrimp fed D2 while were mostly upregulated in shrimp fed D3 and D4. Results suggested that FM could be partially replaced with SBM and PBM in shrimp feeds supplemented with 0.06% AQUAVI® alone or combined with 0.10% ECOBIOL® without adversely affecting the growth and nutrient metabolism of P. vannamei.

Arevalo-Sainz K, Gonzalez-Galaviz J, Casillas-Hernández R, Fraijo-Valenzuela A, Gil-Núñez J, Rodriguez-Anaya L, Lares-Villa F, Gortares-Moroyoqui P, Arias-Moscoso J, Bórquez-López R. Methionine sources and Bacillus amyloliquefaciens CECT 5940 effects on growth, body composition, and nutrient metabolism of Penaeus vannamei fed reduced fishmeal diets. Lat. Am. J. Aquat. Res.. 2024;52(3): 404-415. Available from: doi:10.3856/vol52-issue3-fulltext-3158 [Accessed 19 Jul. 2024].
Arevalo-Sainz, K., Gonzalez-Galaviz, J., Casillas-Hernández, R., Fraijo-Valenzuela, A., Gil-Núñez, J., Rodriguez-Anaya, L., Lares-Villa, F., Gortares-Moroyoqui, P., Arias-Moscoso, J., & Bórquez-López, R. (2024). Methionine sources and Bacillus amyloliquefaciens CECT 5940 effects on growth, body composition, and nutrient metabolism of Penaeus vannamei fed reduced fishmeal diets. Latin American Journal of Aquatic Research, 52(3), 404-415. doi:http://dx.doi.org/10.3856/vol52-issue3-fulltext-3158