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Email the author (Login required) Antimicrobial peptide fractions obtained from pen shell Atrina (Servatrina) maura viscera through enzymatic hydrolysis and their potential applications in aquaculture
Submited: 2026-03-11 01:46:21 | Published: 2026-06-30 22:44:54
DOI: https://doi.org/10.3856/vol54-issue3-fulltext-3636
Abstract
Marine-derived proteins represent an important source of bioactive peptides with antimicrobial potential. This study evaluated peptide fractions obtained from the viscera of the pen shell Atrina (Servatrina) maura through controlled enzymatic hydrolysis using a multi-enzyme system. Enzymatic hydrolysis increased soluble protein content by 1.48-fold compared to crude extracts, indicating efficient proteolytic processing. Size-exclusion chromatography revealed distinct peptide populations with molecular weights below 29 kDa, which were further characterized by SDS-PAGE analysis. Selected peptide fractions showed antimicrobial activity against Gram-negative bacteria, including Enterobacter aerogenes, Salmonella enteritidis, Salmonella typhimurium, Shigella flexneri, and Shigella sonnei. Dynamic growth inhibition assays demonstrated concentration-dependent effects, with up to 80% reduction in bacterial growth at 200 μg mL-1. Fractions enriched in lower molecular weight peptides exhibited stronger inhibitory activity, suggesting a relationship between peptide size and antimicrobial effectiveness. These results indicate that enzymatically derived peptide fractions from A. maura viscera represent a promising source of antimicrobial biomolecules with potential applications in biotechnology and aquaculture.
